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modernmicroscopy
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articles
Evaluation of a Prototype BF-DF-Oblique-Circular Oblique Lighting (BF-DF-Obl-COL) Condenser
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Ted Clarke, Scientific Photographer and Instrument Maker |
This gave us an interference figure for the polyester
film fully filling the rear focal plane or exit pupil of the objective
as shown in Figure 22. The ray diagram in Figure 23 explains why opening
the aperture diaphragm did not illuminate the outer portion of the rear
focal plane of the 60X objective when the condenser height was previously
established to give best Koehler illumination with the 10X objective.
The high NA rays converged below the object focal plane because of uncorrected
spherical aberration and diverged without passing through the field of
the 60X objective. Figure 24 illustrates what happened when the condenser
was raised. The intermediate NA rays now passed around the field of the
objective and converged above. The axial rays still go through the object
field. Figure 25 illustrates what happened when the field diaphragm was
opened fully so that the intermediate NA rays could pass through the object
focal plane along with the high and low NA rays. Figure 26 illustrates
the situation for an aplanatic condenser without spherical aberration.
Lonert indicated the same behavior for an Abbe condenser but provided
no examples or ray diagrams8.
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Figure
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Figure
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Figure
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