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microscopy in the home shop
Constructing a Scanning Light Photomacrography System
| by |
Ted Clarke, Scientific Photographer and Instrument Maker |
| 3/13/2005 (revised 6/10/2006) | 1 | 2 | 3 | NEXT>> |
I was amazed by the scanning light photomacrography
images Jim Gerakaris showed in his Inter/Micro-84 presentation a “Second
Look at Scanning Light Photomacrography”. My own talk on “Method for Calculating
Relative Apertures for Optimizing Diffraction- Limited Depth of Field
in Photomacrography” complemented Jim’s talk by demonstrating the limitations
of conventional photomacrography even with the aperture diaphragm stopped-down
so much that the loss in image detail started to become visible.1
Jim’s talk was my first exposure to the apparently
unlimited depth of field with the Dynaphot scanning light photomacrography
system.2 My article on
photography of fractured parts and fracture surfaces in Volume 12 of the
ASM Handbook includes a description of the Dynaphot and comparison fractographs
taken with and without scanning.3 This article also includes a table and graph based upon my optimum
aperture analysis. The front cover of the Dynaphot sales brochure has
a spectacular scanning light photomacrograph by Darwin Dale of the head
of a fly, shown in Figure 1. The Dynaphot and its operating principles
are well explained on the second page of the brochure, shown in Figures
2 & 3. There is some confusion caused by the brochure’s diagram in
Figure 3, which claims that the specimen is scanned vertically through
a thin sheet of light. The patent drawing by the inventor of the method,
Dan McLachman Jr, clearly shows in Figure 4 that the beam of light from
the slit lamps converges to a minimum thickness where it intersects the
optical axis of the camera objective.
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Figure 1 |
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Figure 2 |
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Figure
3 |
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Figure
4 |
The effect of the illuminating beam converging and
diverging at the edges of the field being recorded and the diffraction
limited minimum beam thickness at the center of the field was the subject
for my later publication of a mathematical analysis of the scanning method
including a photograph and description of a scanning illumination system
of my own design to be used on my very robust and precise photomacrography
stand.4 This stand is
intended for use in photographing parts and fracture surfaces of significant
weight and is based upon restored components of an early twentieth century
bench-lathe. The stand with the completed scanning light system is shown
in Figure 5. A microscope eyepiece adapter with a 10X high eyepoint eyepiece
is shown mounted in place of a parfocal Olympus 35 mm camera back. This
eyepiece adapter was initially intended as an aid in viewing the fine
details and as a relay lens for an eyepiece-mounted Nikon Coolpix 995
digital camera to be used for test exposures prior to final recording
on film. I was pleasantly surprised to find that the test exposures with
the digital camera were of high quality, even with the shutter held open
on B (bulb) setting for 12 second scans. Figure 6 is a scanning light
photomacrograph of the head of a house fly taken with this now completed
system. The 35 mm film camera would record a much larger field area at
somewhat higher resolution than the digital camera.
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Figure
5 |
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Figure
6 |
| 3/13/2005 (revised 6/10/2006) | 1 | 2 | 3 | NEXT>> |
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