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"how to" tutorial series
"How To" Tutorial Series
Universal Stage Use on the Olympus BX51-Pol Microscope
| by |
John Gustav Delly, Scientific Advisor, College of Microscopy, Westmont, IL |
Stage Adapter Plate
With the riser
block finished, the Leitz Universal Stage has to be attached to the Olympus
BX51-Pol stage. On a Leitz polarizing microscope, there are already two drilled and
tapped holes in the pol-stage to accommodate the Universal Stage. These holes
are 85 mm apart, and are tapped M3.5 x 0.60, to take the two
special U-stage mounting screws that accompany the Universal Stage. [Note:
for those not familiar with screw thread designation, the “M” means that the
dimensions are in metric units; the “3.5” means that the thread diameter is 3.5
mm; and the “0.60” refers to the pitch of the thread, i.e. the number of
threads per millimeter.] There are no matching tapped holes 85 mm apart in
the Olympus stage; there are, however, several other tapped holes in the Olympus
stage toward the perimeter that could serve to attach a metal plate, to which the
Leitz U-stage could be mounted. Accordingly, measurements were taken. Figure
5 (Download PDF) is an engineering drawing of the proposed adapter plate, eventually to be
black anodized. The adapter plate can be made from 1/16” thick or 3/32” thick
aluminum sheet. If homemade, the sheet aluminum can be bought at a hardware
store and the necessary piece can be fabricated using a circle-cutter chucked in a
drill press. [A circle-cutter is like a pilot drill with an outboard arm that
carries on its end a cutting tool that can be adjusted for distance along the
arm.]
click image to enlarge (100K)
Figure 5 |
In use, the
universal stage requires that the central disc insert in the microscope stage
be removed; there is a correspondingly large opening in the base of the
U-stage. A 5.83” diameter disc is cut out of the aluminum sheet, and a 2.84”
diameter piece is cut out of the center of that. Two ¼” holes are drilled 120°
apart where specified in the engineering drawing; these will allow room for
attaching, centering, and adjusting the adapter plate to the Olympus stage.
Then two holes are drilled 85 mm apart where indicated in the engineering
drawing. These holes should be made with a 2.9 mm drill, which is the size
needed to tap the two holes M3.5 x 0.60. That completes the adapter plate,
which fastens to the M3.0 x 0.50 tapped holes in the Olympus stage, after the
stage is rotated to zero degrees at the vernier, and locked. The ¼” holes in
the adapter plate allow plenty of room for the 3.0 mm diameter threads for
centering. For convenience, knurled thumb screws may be used for attaching the
adapter plate to the stage, so a screwdriver is not needed. If the stage is equipped with centering screws, centration of the U-stage can be effected that way.
Alternatively,
if the microscopist happens to have a Linkam hot stage, the U-SRP Adapter plate
used to attach the hot stage to the Olympus stage may be drilled and tapped to
take the U-stage. Figure 6 (Download PDF) is an engineering drawing of the Linkam U-SRP
Adapter. Figure 7 is a photo of the actual adapter plate, which has two holes
drilled 85 mm apart—centered top and bottom in the photo—and tapped M3.5 x
0.60.
click image to enlarge (66K)
Figure 6 |
click image to enlarge (49K)
Figure
7 |
Figure 8 shows
the Leitz 4-axis Universal Stage mounted on the modified Linkam adapter, using
two cheese-head screws and washers; the screws are M3.5 x 0.60, or the mounting
screws that accompany the U-stage can be used—with additional washers if the universal stage has a centering base.
click image to enlarge (167K)
Figure 8 |
click image to enlarge (158K)
Figure
9 |
Figure 9 shows
the Leitz 4-axis universal stage mounted on its adapter plate, now affixed to
the Olympus stage using two knurled thumbscrews M3.0 x 0.50; fiber washers were
chosen for smoother sliding during the centering operation. This completes the
U-stage-to-Olympus-stage modification (the microscope stage has been removed
from the microscope for clarity).
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